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The generation of OH provokes the oxidation of PUFAs and thereby initiates the cascade leading to ferroptosis

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Cells were fixed by vortexing with simultaneous addition of Complete Cell Fixation Solution (Fixation Reagent1, ice-cold 100% methanol, Stanlab, Poland) with DEPC, followed by 15 min incubation on ice and then washed with WRS Buffer (Scale Biosciences) and resuspended in WRS Buffer

Vitamin C: Vitamin C, also known as ascorbic acid, is a vital nutrient with powerful antioxidant properties that help protect cells from damage caused by free radicals