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doi: 10.1111/j.1365-2672.2006.02963.x

To this end, we quantified the variability of the results using an analysis of variance approach (ANOVA) following a designed acquisition and analysis procedure outlined next: To quantify ORS speeds perpendicular (ORS) to the hair fiber growth axis, we imaged longitudinally within the ORS layer of the hair follicle (Supplementary Fig

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Then, a final concentration of 10 g proteins was diluted in a 4X Laemmli Loading Buffer (Bio-Rad, Hercules, CA, USA) and subjected to denaturation at a temperature of 95 C for 5 min