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For metabolite extraction, 5 l of urine was transferred to a glass insert and extracted using 195 l of cold 80% methanol spiked with 500 nM metabolomics amino acid standard mix (Cambridge Isotopes Laboratory)

The FlowJo software was used to measure eGFP expression according to the following equation: MFI siGFP indicates the mean fluorescence intensity of cells treated with GFP-siRNA and MFI siCTRL indicates the mean fluorescence intensity of cells treated with negative control-siRNA

The Epichlo endophyte or Se concentration significantly affected the glutathione reductase, reduced glutathione, oxidized glutathione, and the GSH/GSSG ratio over time

Thus, in order to control the progression of DM complications, it is important to control hyperglycemia [24]