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FEV 1 declines naturally with age, but the rate of decline in COPD patients is generally greater than that in normal subjects

Markers of antioxidant status Whole blood aliquots were analyzed for superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities on a microplate reader (BioTek Synergy H1, Agilent Technologies, Santa Clara, CA) via spectrophotometry using commercially available kits (Cayman Chemical Company, Ann Arbor, MI)