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After cells were treated with NAC or Mito 10 -NAC for 24 h, eight baseline OCR measurements were taken before injection of oligomycin (1 mg/mL) to inhibit ATP synthase, dinitrophenol (50 M) to uncouple the mitochondria and yield maximal OCR, and inhibitors of complexes I and III (1 M rotenone and antimycin) to inhibit mitochondrial respiration

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The presence of glycine residues in MRP7s binding site confers localized flexibility, presumably facilitating the binding of a variety of ligands and compensating for the proteins relative overall rigidity in comparison to MRP1