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Description

After 3 months of treatment (when the mice reached the early stage of the disease), TRF-treated Q175 mice showed improved motor activity rhythms and sleepwake cycles

Transfection efficiency was detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blot 48 h after transfection and compared with cells transfected with the corresponding negative control vector or negative control siRNA