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ZDF rat Six- to 7-week-old male obese (fa/fa) Zucker rats (ZUCKER- Lepr fa , Charles River Laboratories) were fed a HFD for a total of 5 weeks and dosed with vehicle (5% DMSO and 0.5% Kollidon-30 in 100 mM sodium phosphate buffer) or TLC-2716 (0.1 or 1 mg per kg (body weight)) once daily by oral gavage for the last 2 weeks