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The fresh tissue was thoroughly homogenised in liquid nitrogen following extraction in ice-cold buffer containing100 mM TrisCl (pH 7.8) buffer containing 10 mM MgCl 2 , 1 mM PMSF, 10 mM DTT, 100 mM EDTA, and 2% PVP, 0.1% protease inhibitor

a total dose of 60 mg/kg/day was well tolerated with no observable adverse effects

See the sources that passed How to Verify a BPC-157 Source Sourcing is where most dosing protocols quietly fail before the peptide ever reaches a syringe

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