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After 16 h of growth, cells were harvested via centrifugation and the resulting supernatant was harvested and sterilized using 0.22 m syringe filter sterilizing units (VWR, 76479-016) to remove residual cells

Formononetin inhibits both the interaction of FGF with FGFR and the downstream signaling of STAT3 and the PI3K/AKT pathway

Some, like Clostridium perfringens , Clostridium tetani , and Clostridium botulinum , which cause gangrene, tetanus, and botulism, respectively, are obligate anaerobes that cannot survive in the presence of oxygen