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More recently, novel antibody-independent methods have been developed that allow m 6 A mapping at single-nucleotide resolution, such as SELECT, which uses a single-base elongation and ligation-based qRT-PCR amplification to identify m 6 A modifications at specific nucleotide locations 34

doi: 10.1073/pnas.95.6.3117 Summary Keywords endothelium, Tat, blood brain barrier, pulmonary vascular remodeling, cardiovascular dysfunction Citation Chandran S, Adler M, Chen L, Kaur S and Dhillon NK (2025) HIV-Tat and vascular endothelium: implications in the HIV associated brain, heart, and lung complications