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Next day, the media was replaced with astrocyte maturation medium supplemented with 10 ng/ml IL-1 (R&D systems 201-LB-025)

Previous studies have suggested that PINK1-dependent mitophagy and chemotherapy-induced transcriptional reprogramming may contribute to acquired resistance after NAC, as exemplified by the association of high PINK1 expression in PostNAC specimens with poor prognosis and by organoid-based analyses showing increased tumorigenicity and EMT/hypoxia-related programs after NAC [10, 11]