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Serine and glycine also charge the folate pool with one-carbon units, including N 10 -formyl-THF, that are ultimately used to produce nucleotides, methionine, and S-adenosylmethionine, the latter of which is the major methyl donor for methyltransferase reactions including those of DNA and histones [62, 63]

as the vesicle pH continues to drop, these lipids become activated, facilitating cargo release into the cytoplasm [7]

Review available methylation, Walsh and functional laboratory testing