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Once inside these cells, the particles disassemble and antigens are loaded onto major histocompatibility complexes for presentation to lymphocytes [185]

Although a better fit could be obtained by considering more detailed factors, the consistency between our simple model calculations and the experimental results confirmed our hypothesis that the optimum nanoparticle size is determined by the competition between permeation through gaps opened in the BBB and excretion from blood circulation

Asn240Lys produced the highest yield with 39 mg per liter of culture, while Asn240Lys/Leu244Arg produced the lowest amount of pure enzyme with 11 mg per liter of culture (Table 1)

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