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b , c , Human ( b ) or mouse ( c ) melanomas were treated in culture with erastin and/or deferoxamine

The sections were rinsed three times with double-distilled water and stained with Oil Red O solution (Sigma, O0625) for 15 min in the dark

Laboratory verification THIRD-PARTY TESTED COA AVAILABLE Independently analysed Every peptide is screened by an accredited independent laboratory

After 24 h, total protein extracts were obtained from whole flies homogenized in standard lysis buffer (20 mM Tris-Cl (pH 7.5), 200 mM NaCl, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40 and EDTA-free Complete Protease Inhibitor Cocktail (Roche Diagnostics))