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Plumbagin ameliorates LPS-induced acute lung injury by regulating PI3K/AKT/mTOR and Keap1-Nrf2/HO-1 signalling pathways

The N-glycosylated form of C H 1 was transiently expressed and secreted from Expi293 cells cultured in suspension as His-Fd, containing a TEV tag between the His-V H and C H 1 domains, and co-expressed with a His-tagged light chain