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(Table 1) Results European POAG GWAS identifies newly associated variants near genes with known roles in eye development and function The analysis of individuals of European ancestry (2,302 POAG cases, 213,774 controls) identified 52 genome-wide significant variants and five distinct loci associated with POAG, consistent with previous GWAS findings related to POAG and visual field loss 24

2.5 Thickness and Water Content Water on the BP surface was absorbed using dry gauze, and the thickness of the BPs was measured using a spiral micrometer ( n = 10)

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Quantification of ATM expression Cultured hair follicle melanocytes were seeded into 8-well chamber slides (~ 10,000 cells/well) and left to attach for at least 24 h, fixed in ice-cold methanol for 10 min, and incubated with blocking buffer of 10% donkey serum (Sigma-Aldrich, UK) for at least 30 min