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NADP+/NADPH is a cofactor pair that provides active sources of protons and electrons and is closely linked with GSSG/GSH

Chemosphere 332 , 138814 (2023)

To increase the protein recovery, the pellet from this step was resuspended in a buffer (containing 7 M urea and 2 M thiourea in 0.4 M triethylammonium bicarbonate at pH 8.5), freeze-thawed, vortexed and centrifuged at 15,000 g for 15 min at 20 C