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Next, Binding Site Identification was done using AutoDock 4 59 wherein blind docking of the substrate in the presence and absence of the cofactor to identify the possible binding site was performed

Iron (Fe 2+ ) generates large amounts of hydroxyl radicals through Fenton and iron-catalyzed Haber-Weiss reactions, catalyzing the generation of ROS, which in turn leads to the development of LP (MacKenzie et al., 2008