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Affinity purification handles can be divided into two groups: (1) peptides or proteins that bind a small ligand immobilized on a solid support, as for instance, the His 6 tag and nickel affinity resins, and (2) tags that bind to an immobilized molecule such as antibodies ( The purification of a target protein using an affinity handle offers several advantages over the conventional chromatographic methodologies, namely: (i) The target protein never interacts directly with the chromatographic resin (Waugh, 2005)

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In a prospective trial of 35 patients with ME/CFS, a logistic regression analysis with stepwise elimination identified six pretreatment characteristics that accurately differentiated DCA responders from non-responders, with an ROC AUC of 0.92 [144]

Vaccine, 30 , 22122219