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Additionally, Glu neurons demonstrate significantly elevated levels of hmCG than GABA neurons, particularly in enhancer regions, indicating a role in active gene expression (Jin et al., 2023)

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2.7 ELISA tests for inflammation marker assay Specific rat ELISA kits from SunLong Biotech Co., Ltd., Hangzhou City, Zhejiang, China (catalog numbers SL0722Ra, SL0411Ra, and SL0537Ra, respectively) were used to measure tumor necrosis factor- (TNF-), interleukin-6 (IL-6), and NF-B in hepatic homogenates, which are key indicators of liver inflammation