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231 Given that altered regulation of Cu, homeostasis has been associated with a variety of disease conditions, future studies are warranted in order to determine the precise mechanism by which Cu imbalance causes cellular damage, thus providing important new information that can be used to design treatments for preventing disease and/or slowing disease progression

Conservative amino acid substitutions include the substitution of an amino acid in one class by an amino acid of the same class, where a class is defined by common physicochemical amino acid side chain properties and high substitution frequencies in homologous proteins found in nature, as determined, for example, by a standard Dayhoff frequency exchange matrix or BLOSUM matrix

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