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Accordingly, our work indicates that addition of N-glycan on Ea-domain prevents the degradation of proIGF-1Ea by the proteasome, probably overcoming the folding limitation of unglycosylated proIGF-1Ea and its entry into the ER-associated degradation (ERAD) pathway 40,41

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It was also indicated that the applicability of IM-MS, which provides rotationally averaged CCSs of molecular ions that can be correlated with conformation, to the study of IDPs is further enhanced by the ability of this technique to examine absolute conformation(s), populations of conformation, and conformational changes (Jurneczko et al., 2012)

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