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After incubation, the culture medium was collected, centrifuged at 2,000 g for 10 min to remove cellular debris, and the supernatant was carefully harvested for subsequent cytokine quantification using enzyme-linked immunosorbent assays (ELISA) kits: Mouse TNF alpha SimpleStep ELISA Kit (ab208348

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Dosage and Administration: Administered via intramuscular or subcutaneous injection, dosage depends on the species, weight, and specific deficiency

(1999) RACK1, a protein kinase C anchoring protein, coordinates the binding of activated protein kinase C and select pleckstrin homology domains in vitro