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The slides were then soaked for 30 min in a solution of diluted (0.3%) hydrogen peroxide (H 2 O 2 ) in phosphate-buffered saline (PBS) to block endogenous peroxidase

99 Folate also enhances regeneration of BH 4 from inactive BH 2 by stimulating dihydrofolate reductase and it chemically stabilizes BH 4

Rather than raising NAD+ systemically (as NMN or NR supplementation does), NNMT inhibition preserves NAD+ specifically in the tissue where metabolic dysfunction is most pronounced a precision approach that appeals to researchers studying tissue-specific metabolic regulation

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