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In reaction mixture without enzyme solution, varying amounts of glutathione (0-300 l of stock solution giving 0-260 M final glutathione concentrations) were reacted with 150 l of monobromobimane solution (final concentration 50 M) in presence of phosphate buffer (3.0 ml) in cuvettes

Mengfan et al

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On the cytoplasmic side, Threonine 40, conserved in MGST1, was the closest residue with 4.4 from the threonine oxygen to one of the fatty acid carboxyl oxygens