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Pharmacol Ther 79(3):179192

As actual level of reaction product accumulated in the tissue section as a result of immunohistochemical reactivity is influenced by lots of factors, all parameters such as gray level adjustment, histogram stretch, and minimal optical density were carefully controlled following the recommended procedures

One of the first chelating agents documented to reduce RS production (specifically the hydroxyl radical) in vitro was diethylenetriaminepentaacetic acid (DTPA)