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Through co-encapsulation, such nanoparticles could release BBB modulators in the local tumor site, which adjust the permeability of BBB in the paracellular pathway to ensure more nanoparticles in the same area

Cells were pelleted by centrifugation (300 x g , 3 min, room temperature in 15-ml conical tubes (Corning), followed by 21,000 x g , 2 min, room temperature in microcentrifuge tubes)

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