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Description

Seasonal variation in the intestinal bacterial flora of hybrid tilapia ( Oreochromis niloticusOreochromis aureus ) cultured in earthen ponds in Saudi Arabia

10.1371/journal.pgen.1007321 84 HirataY

The animals were anesthetized by inhalation route with Isofluorane (Isboa Vet) using a vaporizer and intranasal administration was performed with two instillations of 20 l of the bacterial suspension prepared in PBS