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Following methanol-chloroform precipitation, proteins were denatured and reduced in 6 M urea, 2 M thiourea, and 10 mM tris(2-carboxyethyl) phosphine (TCEP, ThermoFisher)

Schulz TJ, Zarse K, Voigt A, Urban N, Birringer M, Ristow M: Glucose restriction extends Caenorhabditis elegans life span by inducing mitochondrial respiration and increasing oxidative stress

In parallel, capsaicin induces the expression of antimicrobial peptides, such as regenerating islet-derived protein 3 gamma (Reg3), and modulates bile acid metabolism, both of which contribute to maintaining microbial diversity and community stability (120, 143, 145)

S.SchoenenJ.et al (2021)