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To determine ATP levels in samples, add 50 l of each thawed sample or a standard solution of known ATP content to 100 l ice-cold Cell ATP-Releasing Reagent and incubate for 5 min on ice, then add 100 l of ice-cold ATP Assay Mix (1:25 dilution in assay mix buffer)

2.4 Animal model and treatment A cohort of 70 SPF grade C57BL/6J mice and ApoE / mice, aged 810 weeks, male, weighing 2226 g, were provided from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., with license number SCXK (Beijing) 2021-0006

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Collagen Synthesis and Connective Tissue Repair Peptides like BPC-157 and TB-500 stimulate collagen synthesis, supporting the strength and elasticity of tendons, ligaments, and joint structures