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The pooled sample was purified using a high-capacity reverse phase cartridge (Oasis HLB, Waters) and the eluate was fractionated using high pH reverse phase spin columns (Pierce) according to manufacturer specifications, yielding 8 fractions

The FC between the two groups indicated is expressed as log 2 ratio

Among the complex pathophysiological processes and pathogenic mechanisms of IDD, oxidative stress is considered as the main pathogenic factor." - Source