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For example, research utilizing microglial extracellular vesicles (EVs) from cryopreserved AD brain tissue analyzed lipidomic, transcriptomic, and proteomic datasets to illustrate how microglial activity shifts in late-stage AD, impacting the clearance of tau-laden neuronal debris (266)

While DIA typically yields increased peptide identifications compared to DDA, it often produces a lower proportion of confidently localised sites due to the increased spectral complexity, a challenge that is further exacerbated in low-input workflows, where the intensity of diagnostic fragment ions is reduced (Muneer et al., 2024, Kitata et al., 2021)

This is especially important for compounded or injectable preparations

Glycoproteins, as part of the endothelial glycocalyx, have a small cytoplasmic tail, a transmembrane domain and variable extracellular domains [266]