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Studies indicate that cryoinjury during freezing induces abnormal intracellular ROS elevation, disrupts calcium homeostasis, triggers oxidative stress and zona pellucida hardening, and causes abnormal distribution/function of organelles such as mitochondria, the endoplasmic reticulum, and the spindle apparatus (Sun et al., 2023)

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This research firmly establishes oxidative stress as a shared mechanistic basis for these conditions and underscores the critical need for targeted antioxidant therapies to protect cellular infrastructure

Since L-glutamine has a major role in intestinal health, we discussed how L-glutamine might improve tight junction functions, gut microbiome, immunity, and inflammatory conditions in SCD to provide the benefits against VOC