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Measurement of glutathione Total glutathione (GSH) was measured spectrophotometrically in the BALF, plasma, and tissues as previously described [17]

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It plays a critical role in the growth, development, differentiation, synaptic connectivity, and post-injury regenerative repair and protection (especially in the prefrontal and hippocampal areas, which are related to cognitive function) of neurons 41

2-Acetamidophenol upregulates the expression of glutathione synthesis-related genes (such as GCLC, GCLM, GSS ) and iron ion transport genes (such as FPN1, FTH), reduces the accumulation of intracellular reactive oxygen species (ROS) and ferrous ions (Fe 2+ ), and enhances the activity of glutathione peroxidase GPX4 , thereby inhibiting macrophage phagocytosis of oxidized low-density lipoprotein (ox-LDL) and foam cell formation