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After 60 min incubation the samples were washed 2x with 1 ml of binding buffer (125 mM Tris-HCl (pH = 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1% Tween 20), mixed with 1 ug of SNIP1 peptide in 300 ul of binding buffer and rotated for 60 min at 4 C

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There are other, perhaps more common, genetic variants associated with the risk of developing ADincluding the late onset form of the disease (LOAD)such as ApoE4 [521, 522] or those identified more recently (e.g., mutations in CD36 [523], see [524] for review)

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