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Control cultures were exposed to boiled TNR antibodies, while test cultures were incubated with normal TNR antibodies (#217 011

Assessment of antioxidant enzyme activities and oxidative stress markers To assess antioxidant enzyme activities and oxidative stress markers, frozen-thawed spermatozoa of each group (repeated in triplicated) were collected and centrifuged at 800g for 20 min to separate spermatozoa from seminal plasma

These cells, in turn, secrete cytokines and ROS, further enhancing ferroptosis (108)

Mitophagy promotes inflammation In a neonatal cardiomyocyte model of ischemia-like disease, mitophagy has been identified as an activator of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasomes [115]