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For behavioral assessment, rotarod test increased from 2 s (pre-treatment) to 60 s (post-treatment), approaching normal performance

Idiosyncratic DILI On the other hand, most drugs that cause DILI have a much lower and unpredictable risk

Methods Female C57BL/6 mice were orally administered letrozole (LE) to induce PCOS and then randomly divided into groups receiving daily oral administration of 160 mg/kg NAC (PCOS + NAC group), 200 mg/kg metformin (PCOS + Met group), or 0.5% carboxymethyl cellulose (drug solvent) (pure PCOS group) for 12 days

The pellet was re-extracted three times with 400 l cold methanol/water ( 20 C, 80:20 (v/v)) to produce 2 ml of extract