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doi: 10.2147/HMER.S326468 85 SunJZhaoPShiYLiY

Severe loss of quality of life, due to symptoms such as debilitating fatigue, insomnia (B12 is required for synthesis of melatonin), mental illness, and neurological symptoms

Cells overexpressing HRASV12 or cyclin E1 accumulate R-loops during S and G2 phases of the cell cycle To investigate the molecular mechanisms underlying R-loop formation induced by overexpression of HRASV12 or cyclin E1, we used a U2OS T-REx cell line conditionally expressing catalytically inactive RNase H1 fused to green fluorescent protein [RNH1(D210N)-GFP] 32