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Description

The electroporation cuvette was inserted into the Gene Pulser Xcell Total System (Bio-Rad, Hercules, CA, USA) and electroporated under the following conditions: pulse type: square waveforms

For NAD and FAD measurements, stock solutions (1 mM) were serially diluted in PBS and injected at 30 L/min for 180 s per cycle, followed by surface regeneration with 10 mM glycine-HCl (pH 2.0) for 5 min at 10 L/min