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Cellular senescence was assessed by SA--galactosidase staining (SA--gal staining) using senescence -galactosidase Staining Kit (Cell Signaling Technology, Beverly, MA, United States) according to the manufacturers instructions

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The mouse experiments did not include isonitrogenous or amino acidmatched controls, which presents a potential concern for amino acid imbalance

SNX1 and SNX2 proteins can form a heterodimer in Arabidopsis , potentially collaborating in plant retrograde transport