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PMID: 24023285

Two-fold concentrated stock solutions containing either 2 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt, Germany, CAS-2127-03-9, AT-2) (oxidation control), 10 mM Dithiothreitol (Sigma-Aldrich, Darmstadt, Germany, CAS-3483-12-3, DTT) (reduction control), 5 mM reduced glutathione (GSH, Sigma-Aldrich, CAS-70-18-8, pH 7.4 in 1 M HEPES), 5 mM oxidized Glutathione (GSSG, Sigma-Aldrich, CAS-27025-41-8, pH 7.4 in 1 M HEPES) or HEPES were prepared freshly

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The rubber stopper on your peptide vial, the top of the BAC water vialeverything needs to be impeccably clean