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Standard Operating Procedure for Preclinical Preparation of SLU-PP-332 Injection Based on the accumulated knowledge of SLU-PP-332's physicochemical properties and stability considerations, the following standard operating procedure (SOP) has been developed for preclinical preparation: Materials and Equipment SLU-PP-332 active pharmaceutical ingredient (API) PEG 400 (pharmaceutical grade) Ethanol (200 proof, USP grade) 0.1 M Citrate buffer (pH 4.5) Polysorbate 80 (NF grade) Analytical balance (0.1 mg precision) Magnetic stirrer with temperature control Sonicator bath 0.22 m sterile filters Amber glass vials (Type I glass) Preparation Procedure In a clean, low-light environment, accurately weigh the required amount of SLU-PP-332 API

430 Dichloroacetate (DCA), a PDK inhibitor, enhances Krebs cycle flux, preventing myocardial hypertrophy and restoring contractile reserve without altering the expression of key metabolic regulators

However, the purification of the next generation modalities that lack or have modified Fc domain is not straightforward since it precludes efficient binding to most existing protein A resins

2021;18(5):280-296.[DOI] 26