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Since it is difficult to modify intrinsic GSH reactivity in the presence of cellular GST, warheads with reduced reactivity are preferred to minimize the non-specific covalent binding burden on proteins, which correlates with the in-vitro reaction rate of the drug and GSH [105,106]

A major part of the systemic dose will form mixed disulphides together with serum albumin, while a minimal amount of the drug fractions will undergo hepatic metabolism to become disulphides

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