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Active or suspected cancer GHK-Cu has complex effects on cancer biology that remain incompletely understood

All the cell lines were maintained at 37C in Dulbecco's MEM: Ham's nutrient mixture (F12) 1 : 1 (Gibco Biocult, UK) supplemented with 10% foetal calf serum, 50 IU ml 1 penicillin, 50 g ml 1 streptomycin solution, 2 mM L-glutamine, 1 g hydrocortisone and 12.5 ml HEPES buffer (1 M), all from Gibco Biocult, UK

Typical uses include dilution and resuspension of DNA or RNA, preparation of working stocks and wash solutions, buffer base for TE when combined with EDTA, and preparation of samples and gels for electrophoresis