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To analyze copy number of T cells, total RNA was extracted and purified using TRIzol-chloroform-isopropanol protocol

Other relevant deficiencies include: Vitamin B12 essential for red blood cell production and nerve function

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In the TME, despite various ammonia clearance mechanisms, rapidly proliferating cells, particularly tumor and immune effector cells, have a heightened demand for -KG derived from glutamine breakdown to fuel the TCA cycle, leading to substantial free ammonia production (103)