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Following overnight incubation, the remaining biofilm was stained using crystal violet

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The exclusion criteria were: severe or chronic skin inflammation, serious inner or chronic diseases, intake of drugs that can possibly interfere with skin reactions, application of pharmaceutical or skin care products with active ingredients until 10 days before testing, allergies or severe side effects after usage of cosmetic products, sunbath or usage of tanning bed during the study period, known cancer, pregnancy or lactation period, taking of medication with potential effect on hair growth