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To further validate the specificity of the kinase inhibitors we analyzed known substrates of the individual kinases and found good reproducibility in our dataset (Fig

23, # 20, p

The reaction mixture was poured into the dialysis membrane (Molecular weight cutoff 12 kDa) and dialyzed for 3 days in deionized water, the excess EDC.HCl, NHS, and unreacted proline were removed, then the CS-g-P were freeze-dried at -40 C and kept for further reaction