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In practice with GHK-Cu: The copper dose delivered via typical topical or therapeutic administration is modest Short-term use has not been prominently associated with clinically significant zinc depletion Long-term or high-dose use warrants monitoring in patients where zinc balance is clinically important Patients with known zinc deficiency or on zinc-replacement should be monitored more closely A reasonable clinical approach: monitor copper and zinc status in patients on chronic GHK-Cu, particularly in symptomatic or long-duration protocols

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However, the influence of Epithalon on organotypic skin cell cultures proliferation in young and old animals were also investigated