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Microscopic and Ultrastructural Changes of the Intestine in Abdominal Compartment Syndrome

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The 1-cm-long root segments were incubated in 10% KOH at 90C for 50 min, bleached by 10% H 2 O 2 for 15 min, acidified by 0.2 mol/L HCl for 10 min, and stained by 0.05% trypan blue in lactophenol for 30 s

Its use is banned in certain countries (like France) due to fears of a cancer risk