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IGFBP-2 actions are linked to insulin, although only in cases of hyperinsulinemia [173] where it seems to play a role on adipocyte autocrine control [174]

Chen, X., Zeh, H

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After washing with PBS, the sections were reacted with the secondary antibody anti-rabbit-AlexaFluor488 (1:200 in PBS, #A11008, Thermo Fisher Scientific, Waltham, Massachusetts, USA) for 2 h at room temperature